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First, instead of the package's EDTA-that contains elution buffer, ten mM Tris, pH7-8 is Typically used for the final elution, significantly for samples that happen to be being sequenced with PacBio chemistry, given that even small quantities of EDTA can impede downstream procedures A part of sequencing library planning. Nuclease-free drinking wat
Initially, rather than the kit's EDTA-made up of elution buffer, 10 mM Tris, pH7-8 is Generally used for the ultimate elution, particularly for samples that happen to be to become sequenced with PacBio chemistry, considering that even small portions of EDTA can impede downstream procedures included in sequencing library preparing. Nuclease-free dr